Donkey IgG anti-Sheep IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Donkey IgG anti-Sheep IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Artikelnummer: ROC-613-7502
• Hersteller Artikelnummer: 613-7502
• Alternativnummer: ROC-613-7502
• Hersteller: Rockland Immunochemicals
• Wirt: Donkey
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Sheep
• Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
• Konjugation: Alk. Phos.
• Alternative Synonym: Donkey Anti-Sheep IgG alkaline phosphatase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody Alk phos Conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Sheep
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000
• Anwendungsbeschreibung: Anti-Sheep IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

Bilder

ELISA results of purified Anti-SHEEP IgG (H&L) (DONKEY) Alkaline Phosphatase Conjugated tested against Sheep IgG. Each well was coated in 1.0 µg of antigen. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n pNPP Buffer) and Substrate (p/n TMB-8000).