Rabbit IgG anti-Swine IgG (H+L)-Biotin, MinX none

Artikelnummer: ROC-614-4602
Artikelname: Rabbit IgG anti-Swine IgG (H+L)-Biotin, MinX none
Artikelnummer: ROC-614-4602
Hersteller Artikelnummer: 614-4602
Alternativnummer: ROC-614-4602
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Porcine
Immunogen: Swine IgG whole molecule
Konjugation: Biotin
Alternative Synonym: Rabbit Anti Swine IgG Biotin Conjugated Antibody, Rabbit Anti-Swine IgG Biotin Conjugated Antibody, Rabbit Anti-Swine IgG Antibody Biotin Conjugation
Format: IgG
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Swine
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: Anti-Swine IgG Biotin Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-
Western Blot of Biotin conjugated Rabbit anti-Swine antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none.