Anti-Swine IgG Biotin Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Swine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-
Western Blot of Biotin conjugated Rabbit anti-Swine antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none.
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