Goat F(ab)2 anti-Human IgG (Fc)-Alk. Phos., MinX Bo,Ho,Ms,Rt, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Goat F(ab)2 anti-Human IgG (Fc)-Alk. Phos., MinX Bo,Ho,Ms,Rt, ALP, Polyclonal , AP
• Artikelnummer: ROC-709-1517
• Hersteller Artikelnummer: 709-1517
• Alternativnummer: ROC-709-1517
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG F(c) fragment
• Konjugation: Alk. Phos.
• Alternative Synonym: Goat F(ab)2 Anti-Human IgG F(c) Alkaline Phosphatase Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Human IgG Fc Fragment Alkaline phosphatase Conjugated Antibody, Goat F(ab)2 Anti-Human IgG F(c) Fragment Antibody alk phos Conjugated
• Format: F(ab')2
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): Bovine,Equine,Mouse,Rat

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: F(ab)2 Anti-Human IgG F(c) Alkaline Phosphatase Antibody has been tested by dot blot and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature

Bilder

Dot Blot of Goat F(ab)2 Anti-HUMAN IgG F(c) Alkaline Phosphatase Conjugated Antibody Min X Bv Hs Ms & Rt Serum Proteins. Lane 1: 100ng hu IgG. Lane 2: 33.3ng hu IgG. Lane 3: 11.1ng hu IgG. Lane 4: 3.70ng hu IgG. Lane 5: 1.23ng hu IgG. Secondary Antibody: Gt-F(ab)2 anti-Hu IgG Fc Alk Phos at 1µg/mL. Blocking Buffer: BlockOut MB-073 for 30 min at RT.