Anti-Human IgG was produced by repeated immunization with Human IgG F(c) fragment in goat.
Konjugation:
RPE
Alternative Synonym:
Goat F(ab)2 Anti-Human IgG F(c) Phycoerythrin Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Human IgG Fc Fragment Phycoerythrin Conjugated Antibody, Goat F(ab)2 Anti-Human IgG F(c) Fragment Antibody PE Conjugated
Format:
F(ab')2
Spezifität:
IgG (Fc)
Minimale Kreuzreaktivität (MinX):
Bovine,Equine,Mouse,Rat
Klonalität:
Polyclonal
Konzentration:
0.5 mg/mL by absorbance = 82.0 at 565 nm
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
Anwendungsbeschreibung:
F(ab)2 Anti-Human IgG F(c) Phycoerythrin Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background
Western blot of Phycoerythrin conjugated Goat F(ab)2 Anti-Human IgG F(c) Pre-Adsorbed secondary antibody. Lane 1: Human Fc. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human IgG F(c). Other band(s): None.
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