Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This pro
Characterization of the effects of Akt and c-Myc on mitochondria.FL5.12 parental and FL5.12-AM cells were cultured with DOX or 4-OHT for 36 h.(B) cells were incubated with control IgG or anti-Cox IV subunit 1 and then incubated with fluorescein isothiocyanate-conjugated anti-mouse IgG secondary antibody [710-1232]. The expression level of mitochondrial complex IV subunit 1 was analyzed by flow cytometry. Note that the addition of 4-OHT increased the expression of complex IV subunit 1 in FL5.12-AM but not in the parental cells. Fig 3. PMID: 20018866.
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