0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid
Target-Kategorie:
Bovine
Antibody Type:
Primary Antibody
Application Verdünnung:
IF Microscopy: 1:500, WB: 1:5000
Anwendungsbeschreibung:
Anti-Neurofilament H antibody is tested for use in Western Blotting, IHC, and ICC. Specific . Expect a band of approximately 200 kDa in size corresponding to the Neurofilament H proteins in the appropriate cell lysate or extract. Researchers should deter
Western Blot of Anti-Neurofilament H (Mouse) Antibody. Lane 1: rat cortex lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Neurofilament H antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 200kDa/~200kDa for NF-H protein. Other band(s): none.
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