Biotin Alkaline Phosphatase Conjugated

Produktübersicht

• Artikelname: Biotin Alkaline Phosphatase Conjugated
• Artikelnummer: ROC-B000-05
• Hersteller Artikelnummer: B000-05
• Alternativnummer: ROC-B000-05
• Hersteller: Rockland Immunochemicals
• Kategorie: Sonstiges
• Applikation: DOT, ELISA
• Konjugation: Biotin
• Alternative Synonym: Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin

Produkteigenschaften

• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Biotin Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assays target of interest is coupled with streptavidin.

Bilder

Biotin Alkaline Phosphatase Conjugated

Detection of 2 nmol/L of a 48-mer synthetic oligonucleotide target (T) using the Strept2-AP conjugate, a single generation of the Strept2-AP/(biot-AP) assembly, or a single generation of the Strept/(biot-AP)k complex. A noncomplementary oligo (NC) was used as the negative control. The Strept2-AP solution used for the conventional assay and for assembling the Strept2-AP/(biotAP) architectures was 10 µL of 3.1 * 10-15 mol/µL Strept2-AP + 10 mg/mL BSA in DEA buffer, 10 min of interaction. Further details are described in the Experimental Section. Figure 3. PMID: 16618179.

Dot Blot results of Biotin Alkaline Phosphatase Conjugate. Dots are Streptavidin: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Biotin-Alk Phos at 1ug/mL in MB-070 1hr RT. Imaged with BioRad ChemiDoc, Colorimetric filter.