Biotin Glucose Oxidase Conjugated

Produktübersicht

• Artikelname: Biotin Glucose Oxidase Conjugated
• Artikelnummer: ROC-B000-07
• Hersteller Artikelnummer: B000-07
• Alternativnummer: ROC-B000-07
• Hersteller: Rockland Immunochemicals
• Kategorie: Sonstiges
• Applikation: DOT, ELISA
• Konjugation: Biotin
• Alternative Synonym: Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase

Produkteigenschaften

• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Biotin Glucose Oxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assays target of interest is coupled with streptavidin.

Bilder

Biotin Glucose Oxidase Conjugated

Monitoring the interaction of a target with a specific ligand: (a) a microsensors emitted-light intensity at 660 nm during the covalent binding of a 16.7 µM SAv solution with a microsensors bare gold surface, followed by the interaction of a 1 nM bIgG solution with the microsensor-bound SAv. (◯) exponential-decay best-fits to the emitted-light intensities:R2(SAv) = 0.88,R2(bIgG) = 0.84. (b) a microsensors emitted-light intensity at 660 nm during the covalent binding of a 2 µM SAv solution with the microsensors bare gold surface, followed by the interaction of a 10 nM bGOx solution with the microsensor-bound SAv. (◯) exponential-decay best-fits to the emitted-light intensities:R2(SAv) = 0.99,R2(bGOx) = 0.96. (c) a microsensors emitted-light intensity at 660 nm during the interactions of 100 mM DGlu/DPBS (50 mM betaDGlu equivalent) and 100 mM LGlu/DPBS solutions with microsensor-bound bGOx. The specific microsensor was that shown inFigure 5(b). The LGlu solution had the same refractive index as the DGlu solution but did not bind to bGOx. Both DGlu/DPBS and LGlu/DPBS solutions produced fast emitted-light intensity changes on wash-in/wash out because they had a higher refractive index than DPBS. However, only the DGlu/DPBS solution produced a slower change in emitted-light intensity due to formation of betaDGlu - bGOx complex. (d) a microsensors emitted-light intensity at 660 nm during the covalent binding of a 2 µM SAv solution with the microsensors bare-gold surface, followed by the interaction of a 66.7 nM bIgG solution with the microsensor-bound SAv. (◯) exponential-decay best-fits to the emitted-light intensities:R2(SAv) = 0.90,R2(bIgG) = 0.94. The sample flow rate in all experiments was ~0.2 nL/s with a solution temperature of 24.0 0.1 C.*No signal was recorded during sample loading. Figure 5. PMID: 23443397.

Dot Blot of BIOTIN GLUCOSE OXIDASE Conjugated. Lane 1: Biotin Conjugated Glucose Oxidase. Lane 2: Glucose Oxidase. Load: 3-fold serial dilution starting at 200 ng. Primary Antibody: None. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: 1% BSA-TTBS 30 min at RT.