FLAG TAG Immunoprecipitation Kit, Immunoprecipitation Kit for FLAG Antibody, DYKDDDDK, Anti-DYKDDDDK IP Kit, IP FLAG Antibody KIT, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody
Konzentration:
None
Application Verdünnung:
IP: User Optimized
Anwendungsbeschreibung:
Rockland Immunochemicals Anti-DYKDDDDK (FLAG) Kit for Immunoprecipitation is intended to provide a simple, reliable and convenient purification system for recombinant proteins containing the FLAG epitope tag. Immunoprecipitation is a powerful technique
This product is assembled as a kit. See attached protocol or CofA for further details.
Coomassie stained SDS-PAGE using Anti-DYKDDDDK (FLAG) Kit for Immunoprecipitation to purify recombinant proteins containing the FLAG epitope tag from an E.coli cell lysate. Lane 1- cell lysate before immunoprecipitation. Lane 2- cell lysate after immunoprecipitation showing depletion of protein. Lane 3 - purified FLAG -tagged recombinant protein.
Immunoprecipitation and Flow diagram for Anti-DYKDDDDK (FLAG) KitAntibody binding principles are shown for the Anti-DYKDDDDK (FLAG) Kit to purify FLAG - tagged recombinant proteins. The anti-FLAG antibody bound to agarose beads captures the FLAG - tagged recombinant protein from solution. After copious washing with PBS, the buffer condition is changed allowing the release and collection of the highly purified recombinant protein.
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