DyLight(TM) Multiplex 649/488/800 Trio Western Blot Kit

Produktübersicht

• Artikelname: DyLight(TM) Multiplex 649/488/800 Trio Western Blot Kit
• Artikelnummer: ROC-KFA019
• Hersteller Artikelnummer: KFA019
• Alternativnummer: ROC-KFA019
• Hersteller: Rockland Immunochemicals
• Kategorie: Kits/Assays
• Applikation: WB
• Alternative Synonym: Fluorescent western blotting, Multiplex western blotting, multi-color western blotting, fluorescent labelled antibodies, fluorescent imaging, DyLights, dyLight conjugates

Anwendungsbeschreibung

• Anwendungsbeschreibung: This DyLight(TM) Multiplex 649/488/800 Trio Western Blot Kit contains: Goat anti-Mouse IgG (H+L) DyLight(TM) 649, Goat anti-Chicken IgG (H+L) DyLight(TM) 488, Goat anti-Rabbit IgG (H+L) DyLight(TM) 800, Opal pre-stained protein standard, Wash buffer (10X TTBS), bloc

Bilder

Simultaneous detection of three proteins on a single blot using Rockland DyLight(TM)-labeled secondary antibody conjugates. Protein lysates from HeLa (Lane 1), PC12 (Lane 2) and K562 (lane 3) cells and 50ng GFP protein (Lane 4) were run on a gel. The cell lysates were spiked with 50ng, 75ng and 150ng GFP protein. Probing of cell lysates and GFP with anti-alpha-tubulin (mouse), anti-beta-actin (rabbit), and anti-GFP (chicken) followed by DyLight(TM) 649 goat anti-mouse IgG (red), DyLight(TM) 800 goat anti-rabbit IgG (pseudocolored aqua) and DyLight(TM) 488 goat anti-chicken IgG (pseudocolored green) conjugates, and imaged using Syngene G:BOX Imaging System resulted in comparable patterns of detection.

Simultaneous detection of alpha-tubulin and GFP on a single blot using Rockland DyLight(TM)-labeled secondary antibody conjugates. Protein lysates from HeLa (lane 1), PC12 (lane 2) and K562 (lane 3) cells and 100ng GFP protein (Lane 4) were run on a gel. The cell lysates were spiked with 25ng, 50ng and 75ng GFP protein. Probing of cell lysates and GFP with mouse anti-alpha-tubulin and chicken anti-GFP antibodies followed by DyLight(TM) 649 goat anti-mouse IgG (pseudocolored green) and DyLight(TM) 800 goat anti-chicken IgG (red) conjugates, and then imaged using Syngene G:BOX Imaging System resulted in comparable patterns of detection. Lane 5: Rockland Opal Prestained Protein Standard 10-245kDa.