Streptavidin | BIOZOL

Artikelname:	Streptavidin
Artikelnummer:	ROC-S000-01
Hersteller Artikelnummer:	S000-01
Alternativnummer:	ROC-S000-01
Hersteller:	Rockland Immunochemicals
Kategorie:	Sonstiges
Applikation:	SDS-PAGE
Konjugation:	Unconjugated
Alternative Synonym:	SA, S avidin, streptococcus avidin

Konzentration:	0.9 mg/mg streptavidin/lyophilizate (balance is sodium chloride).
NCBI:	00084
UniProt:	P22629
Puffer:	0.15 M Sodium Chloride
Formulierung:	Lyophilized

Application Verdünnung:	ELISA: 1:20,000 - 1:200,000, ChIP: User Optimized, Gel Shift: User Optimized, WB: 1:2000-1:20,000
Anwendungsbeschreibung:	Streptavidin has been tested by SDS-PAGE and is suitable for use as antigen, as a control or standard in assays, and most other immunological methods as well as enzyme conjugates and complexes, Southern blots and other methodologies related to DNA and RN

	Dot Blot Results of Llama IgG2 Isotype control Biotin Conjugated. Llama IgG2 Isotype control Biotin Conjugate (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23. Antibody: Streptavidin (p/n S000-01) at 1:40,000 for 30 mins at RT. Block: BlockOut (p/n MB-073) for 30 mins at RT. Exposure: 1 sec.
	Streptavidin
	alphaE-catenin ABD binds cooperatively to actin filaments. (A) alphaE-catenin is composed of an array of five four-helix bundles (blue-shaded boxes) and a C-terminal five-helix bundle (red box). The beta-catenin/homodimerization region and actin-binding domain are marked. All alphaE-catenin constructs used in this study are defined. (B) Localization of 1 µM GFP alphaE-catenin ABD bound to phalloidin-stabilized filamentous actin (20% Cy3 labeled). Scale bar, 5 µm. (C) Average fluorescence signal of GFP alphaE-catenin ABD bound to single-actin filaments plotted against total concentration of GFP alphaE-catenin ABD. Each data point represents average GFP fluorescence per pixel measured over 100 µm of single actin filaments (2 TIRF flow chambers). Data were fitted to either a Hill equation (black, straight line) or a hyperbolic function (red, dashed line). (D) Kymographs showing 2 nM GFP alphaE-catenin ABD binding and dissociating from the sides of single actin filaments in the absence or presence of 0.5 or 1 µM dark alphaE-catenin ABD. (E-G) Histograms of 2 nM GFP alphaE-catenin ABD dwell times on filamentous actin in the absence (E) or presence (F) of 0.5 µM dark alphaE-catenin ABD or (G) 1 µM dark alphaE-catenin ABD. Inset, curve fit of the 1-cumulative distribution frequency: (E) single-exponential fit (tau1= 70 2 ms,n= 1244 molecules), (F) double-exponential fit (tau1= 88 3 ms [58%)], tau2= 659 15 ms [42%],n= 1289 molecules), and (G) double-exponential fit (tau1= 144 4 ms [64%], tau2= 986 26 ms [36%],n= 1210 molecules). Streptavidin p/n S000-01). Fig 1. PMID: 24068324.
	(A-D): ionized calcium-binding adaptor molecule 1 (Iba1)-stained microglia cells from control (A), pilocarpine(PILO)-treated (B,C) and SZR104 + PILO-treated (D) animals. Scale bars: 10 µm. (E): The average microglia cell areas (cell body and processes) in µm2values on the y-axis. Biotinylated secondary antibodies (1:400) and the signal was detected with peroxidase-labeled streptavidin (1:6000) (p/n S000-01). (n =10, mean SEM) in control, PILO-treated and SZR104 + PILO-treated animals (*p 0.05). Fig 3. PMID: 33297593.
	Multiplex lateral flow detection strips with three detection zones and a positive control zone. Strips tested positive(shown from top to bottom) for Giardia, Entamoeba histolytica, Cryptosporidium, Giardia + Cryptosporidium, Entamoeba histolytica + Cryptosporidium, Giardia + Entamoeba histolytica, and Giardia + Cryptosporidium + Entamoeba histolytica, and no pathogens. Streptavidin-coated gold colloid for lateral flow strips (Streptavidin p/n S000-01). Fig 3. PMID: 26669715.
	SDS-Page of Streptavidin. Lane 1: Molecular weight markers. Lane 2: Streptavidin. Load: 1.0 ug per lane. Predicted/Observed size: The molecular weight of streptavidin is 55,000 daltons. The protein is composed of 4 essentially identical polypeptide chains (homotetramer). This product is chromatographically pure Streptavidin and shows predominantly a single 13.800 dalton band by SDS-PAGE.
	Development of PDMS MB well surface coating to enhance capture cells with affinity for alpha-IgG antibody. FITC-conjugated IgG was added to MB wells coated withastreptavidin + biotinylated alpha-IgG,bbiotinylated alpha-IgG only,cuncoated MBs anddstreptavidin + biotinylated alpha-Transferrin. Quantification of the fluorescent intensity averaged over 12 wells is illustrated in (e). Results indicate that FITC-IgG predominantly binds to the (SA) + b-alpha(IgG) coating.Barsindicate standard error,n=12, *p<0.0001. Streptavidin (p/n S000-01)Fig. 4. PMID: 23358874.
	Optimization of streptavidin and aptamers, each concentration and incubation time repeated three times, respectively: (a) Streptavidin concentration, (b) Strept