Human ROBO1 Knockout A549 Cell Lysate, Clone: [Clone 15], Monoclonal

Produktübersicht

• Artikelname: Human ROBO1 Knockout A549 Cell Lysate, Clone: [Clone 15], Monoclonal
• Artikelnummer: ROC-W09-K01-MT9
• Hersteller Artikelnummer: W09-K01-MT9
• Alternativnummer: ROC-W09-K01-MT9
• Hersteller: Rockland Immunochemicals
• Kategorie: Antikörper
• Applikation: SDS-PAGE, WB
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-ROBO1 antibody, ROBO 1, ROBO-1, hROBO-1, Roundabout homolog 1, Deleted in U twenty twenty, DUTT1, DUTT-1

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 2.0 mg/mL by BCA assay
• Klon-Bezeichnung: [Clone 15]
• NCBI: 9
• UniProt: Q9Y6N7
• Puffer: 1X RIPA Buffer with HALT Protease and Phosphatase Inhibitors
• Quelle: Human
• Formulierung: Liquid (sterile filtered)

Anwendungsbeschreibung

• Application Verdünnung: ELISA: User Optimized, ChIP: User Optimized, IP: User Optimized, WB: User Optimized
• Anwendungsbeschreibung: Human ROBO1 Knockout A549 Cell Lysate has been tested by SDS-PAGE and western blot and is suitable for use in Western blot, ELISA, Immunoprecipitation and ChIP. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared wit

Bilder

Western Blot of Human ROBO Knockout A549 Cell Lysate. Lane 1: Opal Prestained MW Marker (p/n MB-210-0500). Lane 2: A549 ROBO KO Clone 5. Lane 3: A549 ROBO KO Clone 15. Lane 4: A549 ROBO KO Clone 17. Lane 5: A549 ROBO KO Clone 18. Lane 6: A549 ROBO KO Clone 21. Lane 7: A549 ROBO1 KO Bulk. Lane 8: A549 WCL Parental (p/n W09-001-372). Lane 9: MCF-7 WCL (p/n W09-000-360). Load: 35µg lysate/lane. Primary Antibody [Blot A] Anti-ROBO1 (p/n 600-401-692) ~181kDa, [Blot B] stripped and re-probed with Anti-Tubulin (p/n 200-301-880) ~50kDa, at 1µg/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30min at RT. Block: TTBS/Casein at RT. No detection of expected band at ~181kDa is observed in ROBO1 knockout A549 when compared with unmodified A549 cell lysates by Western blot.