Total RNA was prepared from cell line WM165. WM165 is a metastatic human melanoma cell line that displays fibroblastic morphology. This cell line was derived from the same patient as the cell lines WM115, WM266-4, and WM165-1. WM115 cell line originated from the primary tumor, and WM239A, WM165-1 and WM266-4 were from individual lymph-node metastases. This cell line expresses mutant BRAF (V600D), PTEN loss of function including hemizygous deletion, and wild type N-RAS, c-KIT, and CDK4. WM165 cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
RNase free water
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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