Total RNA was prepared from cell line WM209. WM209 is a metastatic human melanoma cell line that forms flat tight colonies in culture medium. This cell line contains a G12A mutation at position 12 of the N-RAS gene. This cell line also expresses PTEN loss of function including homozygous deletion and is wild type for BRAF, c-KIT and CDK4. WM209 cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
RNase free water
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Human melanoma tumor cell pellets for assessment of proteins and their phosphorylation status
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