DNA was prepared from cell line WM239A. WM239A is a metastatic human melanoma cell line with thin elongated fibroblastic morphology. This cell line was derived from the same patient as the cell lines WM115, WM266-4, and WM165-1. WM115 cell line originated from the primary tumor, and WM239A, WM165-1 and WM266-4 were from individual lymph-node metastases. The subject (55-year-old female) displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. WM239A cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM239A cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified Genomic DNA is suitable for a number of molecular biology applications including but not limited to preparation of genomic libraries, PCR templates, DNA sequencing, DNA fingerprinting, and mutation analysis.
Human melanoma tumor cell genomic DNA isolated with genomic DNA miniprep kit
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