DNA was prepared from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells are expected to produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified Genomic DNA is suitable for a number of molecular biology applications including but not limited to preparation of genomic libraries, PCR templates, DNA sequencing, DNA fingerprinting, and mutation analysis.
Human melanoma tumor cell genomic DNA isolated with genomic DNA miniprep kit
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