Total RNA was prepared from cell line WM3311. These cells are known to have mutations in GRIN2A, PRDM1, TERT, and are upregulated in Notch2. These cells are different from many melanoma cell lines in that they lack the common V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This cell line is wild type for BRAF, N-RAS, c-KIT, and CDK4. WM3311 cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
RNase free water
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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