Total RNA was prepared from cell line WM3540. WM3540 is a human metastatic melanoma cell line that displays a pigmented, mesenchymal morphology. This cell line was established from a lymph node metastases of a patient. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses D97H mutation in CDK4 gene and is wild type for N-RAS and c-KIT. WM3540 cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
RNase free water
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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