Total RNA was prepared from cell line WM373. WM373 is a metastatic human melanoma cell line that displays a pigmented morphology and grows in tight adherent dense colonies. This cell line was established from a left upper back metastasis site in a 49-year-old male with level IV superficial spreading melanoma. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. WM373 cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
RNase free water
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
