Total RNA was prepared from cell line WM46. WM46 is a metastatic human melanoma cell line that displays a pigmented fibroblastic morphology. This cell line was established from left chest & abdomen in a 43-year-old female patient with level IV superficial spreading melanoma and a tumor thickness of 1.44. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses PTEN loss of function including hemizygous deletion, and R24C mutation at position 24 in the CDK4 gene. The R24C mutation results in an amino acid substitution at codon 24 in CDK4, from an arginine (R) to cysteine (C). This mutation abolishes the ability of CDK4 to bind to p16(INK4a). WM46 cells produce xenograft tumors when injected into immunocompromised mice.
Konzentration:
200 ng / µl (by UV absorbance at 260 nm)
Puffer:
RNase free water
Quelle:
Human
Formulierung:
Liquid
Anwendungsbeschreibung:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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