Recombinant Human ADARB1 Protein (Flag Tag)

Produktübersicht

• Artikelname: Recombinant Human ADARB1 Protein (Flag Tag)
• Artikelnummer: SCM-A602-310F
• Hersteller Artikelnummer: A602-310F
• Alternativnummer: SCM-A602-310F-10,SCM-A602-310F-50,SCM-A602-310F-500
• Hersteller: SignalChem
• Kategorie: Proteine/Peptide

Produktbeschreibung

Recombinant human ADARB1 (ADAR2L) (2-741) was expressed by baculovirus in Sf9 insect cells using an N-terminal Flag tag.

Zusatzinformationen

Product Description

Recombinant human ADARB1 (ADAR2L) (2-741) was expressed by baculovirus in Sf9 insect cells using an N-terminal Flag tag. The gene accession number is NM_015833.4 (transcript variant 2, canonical sequence).

 

Scientific Background

Adenosine deaminases acting on RNA (ADARs) are enzymes that catalyze the chemical conversion of denosines to inosines in double-stranded RNA (dsRNA) substrates. ADARB1 (ADAR2L – Adenosine Deaminase Acting on RNA 2 Long Isoform) is an RNAediting enzyme that modifies adenosine to inosine in doublestranded RNA, impacting RNA splicing, stability, and translation. It contains double-stranded RNA-binding domains and a catalytic deaminase domain, enabling precise substrate recognition and editing. ADAR2L plays a critical role in the nervous system, with its editing of the GRIA2 mRNA Q/R site being essential for proper AMPA receptor function and neuronal calcium regulation (1). This editing safeguards neurons against excitotoxicity, supporting neural plasticity and development. Dysregulation of ADAR2L activity has been associated with neurological diseases and phsycatric disorders (2). Additionally, altered ADAR2L expression may contribute to cancer progression by disrupting RNA processing (3). Its specificity and critical biological roles make it a potential therapeutic target in neurological and oncological disorders.

References

1. Hajji, H. et al.: ADAR2 enzymes: efficient site-specific RNA editors with gene therapy aspirations. RNA. 2022; 28(10):1281-1297.

2. Iwamoto, K. et al.: Serotonin receptor 2C and mental disorders: genetic, expression and RNA editing studies. RNA Biol. 2009; 6: 248–253.

3. Chen, YB. et al.: ADAR2 functions as a tumor suppressor via editing IGFBP7 in esophageal squamous cell carcinoma. Int J Oncol. 2016;50(2):622–630.

 

Formulation

Recombinant protein stored in 50mM Tris, pH7.5 150mM NaCl, 1mM EDTA, 0.5mg/mL Flagtide, 1mM DTT, 10% glycerol.

 

Storage and Stability

Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Bilder

Recombinant human ADARB1 (ADAR2L) (Cat. No.: A602-310F) shows dose-dependent adenosine deaminase activity towards double-stranded RNA in an ELISA format assay.

The purity of ADARB1 (ADAR2L) was determined to be >85% by densitometry. Calculated MW ~85 kDa. Observed MW ~82 kDa