AKT-IN-28 is an allosteric inhibitor of Akt and a derivative of Shikonin. It effectively binds to Akts allosteric site through hydrophobic and hydrogen bond interactions, with a Kd value of 2.07 µM. AKT-IN-28 significantly inhibits Akt activity, inducing apoptosis in KRAS-mutant colorectal cancer cells, arresting the cell cycle at the G2/M phase, and suppressing cell proliferation, migration, and metabolism.
