Rf470 (Rotor NO.4) TFA is an FMR-probe-D-lysine conjugate (Max Ex: 470 nM, Max Em: 640 nM) that can be covalently integrated into bacterial peptidoglycan. In its free state, Rf470 TFA exhibits weak fluorescence, which significantly increases upon integration into peptidoglycan by transpeptidase catalysis. This fluorescence change allows Rf470 TFA to be used for real-time monitoring of peptidoglycan biosynthesis, detecting transpeptidase activity, and screening antibiotics.
Molekulargewicht:
624.63
CAS Nummer:
[2369899-34-1]
Formel:
C28H31F3N4O7S
Target-Kategorie:
Others|||Antibacterial
T211406
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