(S)-3-amino Blebbistatin, retaining the active stereochemistry of the less stable and more phototoxic (-)-blebbistatin, serves as a refined selective cell-permeable inhibitor of non-muscle myosin II ATPases. This compound efficaciously inhibits the Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB across several species with IC50s ranging from 0.5-5 µM, and exhibits minimal inhibition against smooth muscle myosin (IC50= 80 µM). Its primary functions include obstructing apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. (S)-3-amino Blebbistatin overcomes (-)-blebbistatins limitation of rapid degradation under blue light (450-490 nm) exposure, which generates cytotoxic intermediates, thus posing a challenge for fluorescent live cell imaging applications. The introduction of a 3-amino group to its structure notably reduces fluorescence while maintaining (-)-blebbistatins activity, making it a superior alternative for research purposes.
Molekulargewicht:
307.35
CAS Nummer:
[2097141-18-7]
Formel:
C18H17N3O2
Target-Kategorie:
Others|||Myosin
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