Broussonins A and B, new phytoalexins from diseased paper mulberry. Broussonin A shows estrogenic activity with ligand-binding activity of estrogen receptor, transcriptional activity of estrogen-responsive element-luciferase reporter genes. Broussonin A can significantly inhibit adipocyte differentiation in 3T3-L1 cells as measured fat accumulation using Oil Red O assay, it suppresses lipopolysaccharide (LPS)-stimulated inducible nitric oxide synthase (iNOS) expression at the transcriptional level through modulating NF-iB and down-regulation of the Akt and ERK signaling pathways.
