Sterile 10 mM Histidine buffer (pH 5.5 ) is a widely used biochemical buffer applied in protein purification, vaccine manufacturing, drug development, cell culture, and electrophoretic techniques, Sterile 10 mM Histidine (pH 5.5 buffer) possess strong buffering capacity of histidine, whose imidazole side chain maintains stable acidity, making it ideal for experiments requiring tightly controlled physicochemical conditions, additionally, histidine within this buffer can generate photoreactive derivatives that accumulate dramatically under illumination and serve as primary mediators of tryptophan photo-oxidation which is a key consideration in protein formulation and stability research.
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