| Mca-KKVAPYPME-Dap(Dnp)-NH2 is a fluorogenic peptide substrate of proteasomes, in which the Mca fluorescence is internally quenched by Dap(Dnp). This substrate can be cleaved by mammalian, yeast or archaea activated proteasomes. Upon cleavage, the Mca fluorescence can be measured at excitation/emission wavelengths of 320/405 nm using a plate reader or fluorometer. Due to its long sequence as a nanomer, latent 20S proteasome has very weak activity on hydrolysis of Mca-KKVAPYPME-Dap(Dnp)-NH2, but it can be cleaved by activated proteasomes, including the 26S proteasome. |
