Z-Leu-Leu-Glu-AMC (Z-LLE-AMC)

Produktübersicht

• Artikelname: Z-Leu-Leu-Glu-AMC (Z-LLE-AMC)
• Artikelnummer: UBP-G2100
• Hersteller Artikelnummer: G2100
• Alternativnummer: UBP-G2100-5
• Hersteller: UBPBio, LLC
• Kategorie: Sonstiges

Produktbeschreibung

Z-LLE-AMC is a fluorogenic substrate for the peptidylglutamyl peptide hydrolyzing (PGPH) activity or the caspase-like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.

Produkteigenschaften

• Molekulargewicht: 664.8 g/mol
• Puffer: powder, no buffer
• Quelle: chemical synthesis