Linear polyUb Chain-binding Protein Identification / Validation Kit

Produktübersicht

• Artikelname: Linear polyUb Chain-binding Protein Identification / Validation Kit
• Artikelnummer: UBP-J2520
• Hersteller Artikelnummer: J2520
• Alternativnummer: UBP-J2520-1
• Hersteller: UBPBio, LLC
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human

Produktbeschreibung

This kit is designed for two purposes: 1) identification of proteins that specially bind linear polyUb chains in whole cell or tissue lysates, 2) validation of direct interactions between linear polyUb chains and their binding proteins. The non-cleavable 6xHis-tagged linear Ub6 is resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind linear polyUb chains. After binding, 6XHis-tagged non-cleavable linear Ub6 and the binding proteins can be enriched with Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with TEV protease (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.

Produkteigenschaften

• Puffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
• Quelle: E. coli recombinant