Nedd8 Conjugation Kit

Produktübersicht

• Artikelname: Nedd8 Conjugation Kit
• Artikelnummer: UBP-J3150
• Hersteller Artikelnummer: J3150
• Alternativnummer: UBP-J3150-1
• Hersteller: UBPBio, LLC
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human

Produktbeschreibung

Nedd8 Conjugation Kit is designed for assaying the conjugation of Nedd8 to protein substrates in vitro. In a protein neddylation reaction, Nedd8 is first activated by the Nedd8 activating enzyme APPBP1/UBA3 (E1) in an ATP hydrolysis-dependent manner, activated Nedd8 is then transferred to the Nedd8 conjugating enzyme Ube2M (UbcH12) (E2) by formation of a thiolester bond with the catalytic cysteine residue of UbcH12I, Nedd8-charged Ube2M (UbcH12) can mediate protein neddylation in the presence of an appropriate Nedd8 ligase (E3).

Produkteigenschaften

• Puffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
• Quelle: E. coli recombinant