Ubiquitin Linkage Screening Kit I

Produktübersicht

• Artikelname: Ubiquitin Linkage Screening Kit I
• Artikelnummer: UBP-J3200
• Hersteller Artikelnummer: J3200
• Alternativnummer: UBP-J3200-1
• Hersteller: UBPBio, LLC
• Kategorie: Kits/Assays
• Spezies Reaktivität: Human

Produktbeschreibung

Ubiquitin Linkage Screening Kit is designed for two purposes: 1) to identify the ubiquitin lysine residue that is used to link the polyubiquitin chain conjugated on a protein substrate, 2) to determine whether a protein substrate is polyubiquitinated or conjugated with multiple monoubiquitin. A typical 20 µl reaction contains 100 nM UBE1, 2 µM E2 (not supplied), 50 µM Ub or Ub mutants, 4 mM ATP, 2 µM E3 (not supplied) and 2 µM substrate (no supplied) in the ubiquitination buffer.

Produkteigenschaften

• Puffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
• Quelle: E. coli recombinant