Rapid 26S Proteasome Purification Kit-S

Artikelnummer: UBP-J4310
Artikelname: Rapid 26S Proteasome Purification Kit-S
Artikelnummer: UBP-J4310
Hersteller Artikelnummer: J4310
Alternativnummer: UBP-J4310-1
Hersteller: UBPBio, LLC
Kategorie: Sonstiges
Spezies Reaktivität: Human
The Rapid 26S Proteasome Purification Kit is designed to rapidly purify the endogenous 26S proteasome from cell or tissue extracts within 8 hours according to a published method (reference 1) with modifications. This approach uses the N-terminal ubiquitin-like (Ubl) domain of human RAD23B as an affinity bait, which allows the rapid and gentle isolation of endogenous 26S proteasomes. The bound 26S proteasome is subsequently eluted using the C-terminal two ubiquitin-interacting motifs (UIM) of human S5a
Puffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Quelle: E. coli recombinant