Unconjugated (Free) Polyubiquitin Chain Capture Kit

Artikelnummer: UBP-J4420
Artikelname: Unconjugated (Free) Polyubiquitin Chain Capture Kit
Artikelnummer: UBP-J4420
Hersteller Artikelnummer: J4420
Alternativnummer: UBP-J4420-1
Hersteller: UBPBio, LLC
Kategorie: Kits/Assays
Spezies Reaktivität: Human
The Unconjugated (Free) Polyubiquitin Chain Capture Kity enrich free polyUb chains in cell or tissue extracts within 6 hours. This approach uses the N-terminal ZnF-UBP domain of the deubiquitinating enzyme Usp5 to specifically capture free polyUb chains. The ZnF-UBP domain of Usp5 binds the C-terminal diglycine motif in free Ub chains, it does not bind polyUb chains conjugated on substrate proteins
Puffer: 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Quelle: E. coli recombinant