Protein G magnetic resins contain a recombinant protein G covalently coupled on non-porous magnetic polymer resins. Recombinant protein G only contains the two Fc-binding domains of protein G, that can bind to antibodies from various mammalian species, including human, mouse, rabbit, pig, goat, sheep and dog. Protein G magnetic resins can be used for isolating antibodies from serum, cell culture supernatants or ascites, and for immunoprecipitation and co-immunoprecipitation of antigens from cell lysates or tissue extracts. Comparing to agarose-based resins, non-porous magnetic polymer resins have much less non-specific binding, and suitable for rapid isolation and elution using a magnetic stand.
Puffer:
Resuspended in 1X PBS, 0.01% Tween-20, 0.02% Sodium azide (NaN3) at a concentration of 10 mg/ml.
Quelle:
Chemical synthesis
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