Time resolved (TR)-FRET offers a sensitive, low background, stable and homogenous assay for determination of protein ubiquitination. Terbium-ubiquitin and fluorescein-ubiquitin are a pair of modified ubiquitin suitable for TR-FRET assays to assess formation of polyubiquitin chains, thereby reflecting the activity of E2 or E3 enzyme in the reaction. The concentration and molar ratio of terbium-ubiquitin and fluorescein-ubiquitin in the Terbium/Fluorescein Ubiquitin Mix are optimized to achieve excellent signal-to-background ratio in TR-FRET assays monitoring E3 autoubiquitination. Optimization of protein concentrations are often necessary to achieve a desirable signal-to-background ratio. Typical protein concentration ranges are: UbE1 (10-30 nM), E2 (25-250 nM) and E3 (10-250 nM).
Molekulargewicht:
9.5 kDa/9.0 kDa
Puffer:
See Datasheet
Quelle:
E. Coli Recombinant
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