4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP), Rabbit
Artikelnummer:
USB-0004-18
Hersteller Artikelnummer:
0004-18
Alternativnummer:
USB-0004-18-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IP, WB
Immunogen:
Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. MW of Antigen: 15-20kD.
4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation (1). The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 (2,3). 4E-BP1 is phosphorylated in vivo on multiple residues (4), phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70 (5). Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
