14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor prot

Produktübersicht

• Artikelname: 14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor prot
• Artikelnummer: USB-0014-02-FITC
• Hersteller Artikelnummer: 0014-02-FITC
• Alternativnummer: USB-0014-02-FITC-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FLISA, WB
• Immunogen: Synthetic peptide corresponding to 20aa from the N-terminus of the human 14-3-3 beta/alpha protein. Sequence Homology: delta and zeta isoforms: single aa change, tau isoform: three aa change, gamma isoform: four aa change.

Produktbeschreibung

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and inuence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly inuenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function. Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity. Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Produkteigenschaften

• Reinheit: Purified by immunoaffinity chromatography.
• Formulierung: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).