14-3-3, gamma, Recombinant, Human, aa1-247 (Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG)
Artikelnummer:
USB-0014-07F
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| Artikelname: | 14-3-3, gamma, Recombinant, Human, aa1-247 (Protein Kinase C Inhibitor Protein 1, KCIP-1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase Activation Protein, gamma Polypeptide, YWHAG) |
| Artikelnummer: | USB-0014-07F |
| Hersteller Artikelnummer: | 0014-07F |
| Alternativnummer: | USB-0014-07F-100,USB-0014-07F-500 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms B, Y, E, O, Z, T, and n proteins that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human 14-3-3 Y was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa1-247 of human 14-3-3 gamma expressed in E. coli. Uniprot/Accession: P61981 Molecular Weight: ~28kD (247aa) confirmed by MALDI-TOF Amino Acid Sequence: MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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