| The Activated Protein C (APC) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APC in mouse plasma. Detection Range: 78-5000pg/ml Sensitivity: 33pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *023105A: Microtiter Strips, 1x96 wells Pre-coated, ready to use *023105B: Standard, 2x1 vial 023105C: Standard Diluent, 20ml *023105D: Detection Reagent A (green), 1x120ul *023105E: Detection Reagent B (red), 1x120ul 023105F: Assay Diluent A, 1x12ml 023105G: Assay Diluent B, 1x12ml 023105H: TMB Substrate, 1x9ml *023105J: Positive Control, Lyophilized, 940pg/ml, 1x1vial 023105K: Stop Solution, 1x6ml 023105L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (023105K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023105A, *023105B, *023105D, *023105E and *023105J at -20C. After reconstitution of *023105J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 023105J: Positive Control: Intended Use: The control vials are from a mouse plasma sample known to contain Activated Protein C It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contains mouse APC in mouse plasma (lyophilized powder). Note: The mouse source material used in this product is from healthy mouse of SPF standard (specific pathogen-free animals). Expected Values: 940pg/ml Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentmouseion along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substmousee. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately. |
