Carbonic Anhydrase II (CA2) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Carbonic Anhydrase II (CA2) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-023901
• Hersteller Artikelnummer: 023901
• Alternativnummer: USB-023901-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Carbonic Anhydrase II (CA2) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CA2 in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Detection Range: 3.12-200ng/ml Sensitivity: 1.34ng/ml Intra-Assay: CV10% Inter-Assay: CV12% Kit Components: *023901A: Microtiter Plate, 1x96wells, Pre-coated, ready to use *023901B: Standard, 2x1vial 023901C: Standard Diluent, 1x20ml *023901D: Detection Reagent A, 1x120ul *023901E: Detection Reagent B, 1x120ul 023901F: Assay Diluent A, 2x, 1x6ml 023901G: Assay Diluent B, 2x, 1x6ml 023901H: TMB Substrate, 1x9ml *023901J: Positive Control, 18.92ng/ml, 1x1vial 023901K: Stop Solution, 1x6ml 023901L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (023594K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023901A, *023901B, *023901D, *023901E and 023901J at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450nm immediately.