Carboxypeptidase A4 (CPA4) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Carboxypeptidase A4 (CPA4) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-023920
• Hersteller Artikelnummer: 023920
• Alternativnummer: USB-023920-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Carboxypeptidase A4 (CPA4) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CPA4 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.065ng/ml Intra-Assay CV: < 10% Inter-Assay CV: < 12% Kit Components: *023920A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *023920B: Standard, 2x1 vial 023920C: Standard Diluent, 1x20ml *023920D: Detection Reagent A (green), 1x120ul *023920E: Detection Reagent B (red), 1x120ul 023920F: Assay Diluent A, 1x12ml 023920G: Assay Diluent B, 1x12ml 023920H: TMB Substrate, 1x9ml 023920K: Stop Solution, 1x6ml 023920L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (023920K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023920A, *023920B, *023920D and *023920E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.