Collagen Type II (COL2) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Collagen Type II (COL2) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-024272
• Hersteller Artikelnummer: 024272
• Alternativnummer: USB-024272-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Collagen Type II (COL2) BioAssay(TM) ELISA Kitis a sandwich enzyme immunoassay for in vitro quantitative measurement of COL2 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 93.75-6000pg/ml Sensitivity: 34.82pg/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *024272A: Microtiter Strips, 1x96wells, Pre-coated, ready to use *024272B: Standard, 2x1vial 024272C: Standard Diluent, 1x20ml *024272D: Detection Reagent A (green), 1x120ul *024272E: Detection Reagent B (red), 1x120ul 024272F: Assay Diluent A, 1x12ml 024272G: Assay Diluent B, 1x12ml 024272H: TMB Substrate, 1x9ml *024272J: Positive Control, xxxpg/ml, 1x1vial (Lyophilized) 024272K: Stop Solution, 1x6ml 024272L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (024272K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024272A, *024272B, *024272D, *024272E and *024272J at -20C. After reconstitution of *024272J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.