Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, CSF2) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, CSF2) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-024319
• Hersteller Artikelnummer: 024319
• Alternativnummer: USB-024319-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GM-CSF in human serum, plasma other biological fluids. Detection Range: 15.6-1,000pg/ml Sensitivity: 6.4pg/ml Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to human GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for human GM-CSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GM-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of GM-CSF in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *024319A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *024319B: Standard, 2x1vial 024319C: Standard Diluent, 1x20ml *024319D: Detection Reagent A, 1x120ul *024319E: Detection Reagent B, 1x120ul 024319F: Assay Diluent A (2x),1x6ml 024319G: Assay Diluent B (2x), 1x6ml 024319H: TMB Substrate, 1x9ml 024319K: Stop Solution, 1x6ml 024319L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *024319A, *024319B, *024319D and *024319E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul 024323H: TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul 024323K: Stop Solution. Read at 450nm immediately.