Complement Component 5a (C5a) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Complement Component 5a (C5a) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-024360
• Hersteller Artikelnummer: 024360
• Alternativnummer: USB-024360-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Complement Component 5a (C5a) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of C5a in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 78-5000pg/ml Sensitivity: 31pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *024360A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use *024360B: Standard, 2x1 vial 024360C: Standard Diluent, 1x20ml *024360D: Detection Reagent A, 1x120ul *024360E: Detection Reagent B, 1x120ul 024360F: Assay Diluent A, 1x12ml 024360G: Assay Diluent B, 1x12ml 024360H: TMB Substrate, 1x9ml 024360K: Stop Solution, 1x6ml 024360L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (024360K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024360A, *024360B, *024360D and *024360E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450nm immediately.