Connective Tissue Growth Factor (CTGF) BioAssay(TM) ELISA Kit (Mouse)

Produktübersicht

• Artikelname: Connective Tissue Growth Factor (CTGF) BioAssay(TM) ELISA Kit (Mouse)
• Artikelnummer: USB-024399
• Hersteller Artikelnummer: 024399
• Alternativnummer: USB-024399-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Connective Tissue Growth Factor (CTGF) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTGF in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 62.5-4000pg/ml Sensitivity: 29.6pg/ml Intra-Assay: CV <10% Inter-Assay: CV <12% Kit Components: *024399A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *024399B: Standard, 2x1 vial 024399C: Standard Diluent, 1x20ml *024399D: Detection Reagent A (100X), 1x120ul *024399E: Detection Reagent B (100X), 1x120ul 024399F: Assay Diluent A (2X), 1x12ml 024399G: Assay Diluent B (2X), 1x12ml 024399H: TMB Substrate, 1x9ml 024399K: Stop Solution, 1x6ml 024399L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (024399K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024399A, *024399B, *024399D and *024399E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.