Cytochrome P450 Reductase (CPR) BioAssay(TM) ELISA Kit (Rat)

Produktübersicht

• Artikelname: Cytochrome P450 Reductase (CPR) BioAssay(TM) ELISA Kit (Rat)
• Artikelnummer: USB-024563
• Hersteller Artikelnummer: 024563
• Alternativnummer: USB-024563-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Cytochrome P450 Reductase (CPR) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of rat CPR in tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/ml Sensitivity: 0.107ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *024563A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *024563B: Standard, 2x1vial 024563C: Standard Diluent, 1x20ml *024563D: Detection Reagent A (green), 1x120ul *024563E: Detection Reagent B (red), 1x120ul 024563F: Assay Diluent A, 1x12ml 024563G: Assay Diluent B, 1x12ml 024563H: TMB Substrate, 1x9ml *024563J: Positive Control, xxng/ml, 1x1vial (Lyophilized) 024563K: Stop Solution, 1x6ml 024563L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (024563K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024563A, *024563B, *024563D, *024563E and *024563J at -20C. After reconstitution of *024563J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.