Dermcidin (DCD) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of DCD in human tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.781-50ng/ml Sensitivity: 0.32ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *024634A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *024634B: Standard, 2x1vial 024634C: Standard Diluent, 1x20ml *024634D: Detection Reagent A (green), 1x120ul *024634E: Detection Reagent B (red), 1x120ul 024634F: Assay Diluent A, 1x12ml 024634G: Assay Diluent B, 1x12ml 024634H: TMB Substrate, 1x9ml 024634K: Stop Solution, 1x6ml 024634L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (024634K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024634A, *024634B, *024634D and *024634E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
