Glucagon (GC) BioAssay(TM) ELISA Kit (Rat)

Produktübersicht

• Artikelname: Glucagon (GC) BioAssay(TM) ELISA Kit (Rat)
• Artikelnummer: USB-025290
• Hersteller Artikelnummer: 025290
• Alternativnummer: USB-025290-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Glucagon (GC) BioAssay(TM) ELISA Kit (Rat) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GC in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 18.52-5000pg/ml Sensitivity: 7.45pg/ml. Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *025290A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *025290B: Standard, 2x1 vial 025290C: Standard Diluent, 1x20ml *025290D: Detection Reagent A (green), 1x1vial *025290E: Detection Reagent B (red), 1x120ul 025290F: Assay Diluent A, 1x12ml 025290G: Assay Diluent B, 1x12ml 025290H: TMB Substrate, 1x9ml 025290J: Positive Control, 389.9pg/ml 1x1 vial 025290K: Stop Solution, 1x6ml 025290L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (025290K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Notes on 025290J Positive Control: Intended Use: The control vial is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contains rat glucagon in rat serum (lyophilized powder). Note: The rat based serum used in this product are from healthy rat of SPF standard (Specific Pathogen-Free animals). Expected Values: 389.9pg/ml Usage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Storage and Stability: Store *025290A, *025290B, *025290D, *025290E and *025290J at -20C. Store all the other components at 4C. After reconstitution of *025290J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450nm immediately.