Procollagen I C-Terminal Propeptide (PICP) BioAssay(TM) ELISA Kit (Rat)

Produktübersicht

• Artikelname: Procollagen I C-Terminal Propeptide (PICP) BioAssay(TM) ELISA Kit (Rat)
• Artikelnummer: USB-027592
• Hersteller Artikelnummer: 027592
• Alternativnummer: USB-027592-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Procollagen I C-Terminal Propeptide (PICP) BioAssay(TM) ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PICP in rat serum, plasma and other biological fluids. Detection Range: 1.25-80ng/ml Sensitivity: 0.47ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027592A: Microtiter Strip, 1x96 wells *027592B: Standard, 2x1vial 027592C: Standard Diluent, 1x20ml *0027592D: Detection Reagent A (green), 1x120ul *027592E: Detection Reagent B (red), 1x120ul 027592F: Assay Diluent A, 1x12ml 027592G: Assay Diluent B, 1x12ml 027592H: TMB Substrate, 1x9ml 027592K: Stop Solution, 1x6ml 027592L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (027592K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027592A, *027592B, *027592D and *027592E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul 027590H: TMB Substrate. Incubate 15-25 minutes at 37C. 7. Add 50ul 027590K: Stop Solution. Read at 450nm immediately.