UPK3A belongs to the uroplakin-3 family. Uroplakins (UP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (UPII, UPIIIa, and UPIIIb) or four times (UPIa and UPIb, both belonging to the tetraspanin family). All have large luminal/extracellular domains, but only UPIIIa and UPIIIb have significant cytoplasmic portions in their C-termini. UPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia. Recombinant corresponding to aa19-207 from human UPK3A, fused to His-tag at N-terminal expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG Molecular Weight: ~23.1kD (MALDI-TOF) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
